RNA

Part:BBa_K1941003:Design

Designed by: Dimitri Coukos   Group: iGEM16_EPFL   (2016-10-13)


scCyc1_PP7


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 180
    Illegal NgoMIV site found at 209
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The overall structure of the scRNA with PP7 hairpin was taken form "Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds" J.G.Zalatan et al., Cell 2015, but the 20 base pairs specificity sequence was replaced with a sequence targeting CYC1 in the c6 region of the promoter, as described in "Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas", F. Farzadfard et al., ASC Synth Biol. 2013.

Source

some source!

References